CST, 88855S, Phospho-SUMO-1 (Ser2) (F9B6U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SUMO-1 (Ser2) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SUMO-1 (Ser2) (F9B6U) Rabbit Monoclonal Antibody (CST #88855) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-SUMO-1 (Ser2) (F9B6U) Rabbit Monoclonal Antibody recognizes endogenous levels of SUMO-1 protein only when phosphorylated at Ser2. This antibody may recognize other proteins modified with phospho-SUMO-1 (Ser2) (e.g., SUMO-1-RanGAP at 80 kDa) and a non-phospho protein of unknown origin at 25 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2 of human phospho-SUMO-1 protein. Background Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11). Serine 2 of SUMO-1 was shown to be phosphorylated in cells using mass spectrometry analyses (12). This phosphorylation site was also identified in yeast, , and , suggesting an evolutionary conserved function for this modification (12,13). Alternate Names DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1; OFC10; PIC1; SENP2; Sentrin; small ubiquitin like modifier 1; Small ubiquitin-related modifier 1; SMT3; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3C; SMT3H3; SUMO-1; SUMO1; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG