CST, 8886S, PICH (D4G8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PICH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PICH (D4G8) Rabbit Monoclonal Antibody (CST #8886) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PICH (D4G8) Rabbit Monoclonal Antibody recognizes endogenous levels of total PICH protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly610 of human PICH protein. Background PICH is a helicase of the SNF2 family of ATPases and is essential for proper chromosome segregation during mitosis (1). While PICH was originally proposed to participate in spindle assembly checkpoint signaling (1), that function was subsequently called into question (2). When phosphorylated at Thr1063 by CDK1, PICH binds the polo-box domain of the mitotic kinase PLK1 (1) and targets it to chromosome arms (3), where it appears to facilitate proper chromosome arm cohesion (4). PICH is also a substrate of PLK1 (1). Localized to the cytoplasm during interphase, PICH begins to accumulate at centromeres and kinetochores in prometaphase (4). As chromosomes begin to separate at the onset of anaphase, PICH associates with ultrafine threads between sister centromeres thought to be composed of entangled DNA (5), a natural consequence of DNA replication. PICH is proposed to cooperate with BLM, a RecQ-like helicase implicated in the genetic disorder Bloom's Syndrome, to displace centromeric histones along these threads, thus enabling them to span large distances without breaking (6). This provides a temporal window for topoisomerase IIα-mediated disentanglement (7). Defects in PICH or BLM disrupt proper chromatid segregation and result in the formation of micronuclei (6). Alternate Names ATP-dependent helicase ERCC6-like; DNA excision repair protein ERCC-6-like; ERC6L; ERCC excision repair 6 like, spindle assembly checkpoint helicase; ERCC6L; excision repair cross-complementation group 6 like; excision repair cross-complementing rodent repair deficiency complementation group 6 - like; excision repair cross-complementing rodent repair deficiency, complementation group 6-like; excision repair protein ERCC6-like; FLJ20105; MGC131695; PICH; PLK1-interacting checkpoint helicase; RAD26L; SNF2/RAD54 family protein; Tumor antigen BJ-HCC-15 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 175 Source/Isotype: Rabbit IgG