CST, 8888T, Phospho-Survivin (Thr34) (D2E11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Survivin (Thr34) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Survivin (Thr34) (D2E11) Rabbit Monoclonal Antibody (CST #8888) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Survivin (Thr34) (D2E11) Rabbit Monoclonal Antibody recognizes endogenous levels of survivin protein only when phosphorylated at Thr34. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr34 of human survivin protein. Background Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). Alternate Names API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC5; EPR-1; IAP4; Survivin; survivin variant 3 alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16-18 Source/Isotype: Rabbit IgG