CST, 88925T, TMEM119 (E3E1O) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying TMEM119. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM119 (E3E1O) Horse Chimeric Monoclonal Antibody (CST #88925) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity TMEM119 (E3E1O) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total TMEM119 protein. Species Reactivity: Mouse Source / Purification This recombinant chimeric antibody is engineered from TMEM119 (E3E1O) Rabbit mAb #90840 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, TMEM119 (E3E1O) Rabbit mAb #90840, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM119 protein. Background Transmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). DAM are conserved in human and mouse and have been identified in Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP/PS1 mouse models (3,4). This protein's specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cell types in neurodegenerative disease models (1,2). Alternate Names OBIF; Osteoblast induction factor; TM119; TMEM119; Transmembrane protein 119 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4