CST, 88964S, Glutaminase-1/GLS1 Antibody

Polyclonal Antibody for studying Glutaminase. Validated for Western Blotting. Highly specific and rigorously validated in-house, Glutaminase-1/GLS1 Antibody (CST #88964) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Glutaminase-1/GLS1 Antibody recognizes endogenous levels of total glutaminase-1/GLS1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly116 of human glutaminase-1/GLS1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glutaminase catalyzes the conversion of glutamine to glutamate, the first and rate-limiting step of glutaminolysis (1). Both kidney-type glutaminase (GLS1) and liver-type glutaminase (GLS2) are found in mammals (2). GLS1-mediated glutathione synthesis plays an essential role in redox homeostasis and contributes to increased survival of postimplantation bone cells preconditioned to the hypoxic and ischemic environment in the bone defect site (3). In addition, - -mutant LUAD ( -mutant lung adenocarcinoma) tumors are dependent on increased glutaminolysis (1). Furthermore, recent studies showed higher glutaminolysis and glucose production from glutamine in human primary hepatocytes with gain-of-function missense mutations (4). These findings suggest GLS1 and GLS2 as potential targets in the therapy of bone regeneration and in the treatments of diseases such as cancer and hyperglycemia, respectively (1,3,4). Alternate Names AAD20; CASGID; DKFZp686O15119; EIEE71; FLJ10358; GAC; GAM; GDPAG; GLS; GLS1; GLSK; glutaminase; glutaminase C; Glutaminase kidney isoform, mitochondrial; Glutaminase kidney isoform, mitochondrial 65 kDa chain; Glutaminase kidney isoform, mitochondrial 68 kDa chain; glutaminase, phosphate-activated; K-glutaminase; KGA; KIAA0838; L-glutamine amidohydrolase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-65 SOURCE: Rabbit