CST, 88970T, Desmoglein 2 (8E5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Desmoglein-2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Desmoglein 2 (8E5) Mouse Monoclonal Antibody (CST #88970) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Desmoglein 2 (8E5) Mouse Monoclonal Antibody recognizes endogenous levels of total desmoglein 2 protein. This antibody does not cross-react with desmoglein 1 or desmoglein 3 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human desmoglein 2 protein. Background Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, which are intercellular junctions that mediate strong cell-cell adhesion and are linked to the intermediate filament cytoskeleton. Dysregulation of desmosomal protein expression affects both cell adhesion and communication, and has been shown to result in various human pathologies (1). Desmoglein 2 (DSG2) overexpression promotes lung cancer cell growth (2), and its expression is correlated with metastasis in squamous cell carcinoma of the skin (3). Alternate Names ARVC10; ARVD10; Cadherin family member 5; CDHF5; CMD1BB; desmoglein 2; Desmoglein-2; DSG2; HDGC; MGC117034; MGC117036; MGC117037 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Mouse IgG1