CST, 88989P, SimpleChIP® Universal qPCR Master Mix

Buffer for studying in the research area. Storage This product is stable for 12 months when stored at -20°C and protected from light. It is stable for up to 30 freeze/thaw cycles and can be stored at 4°C, protected from light, for up to 1 month. Specificity / Sensitivity Species Reactivity: All Species Expected Background Dye-based quantitative PCR (qPCR) uses real-time fluorescence of a double-stranded DNA (dsDNA) binding dye, most commonly SYBR Green I, to measure DNA amplification as it occurs during each cycle of PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle, or C value, can be determined. C values can be used to evaluate relative target abundance between two or more samples, or to calculate absolute target quantities in reference to an appropriate standard curve, derived from a series of known dilutions. qPCR is commonly used to detect and quantify target genes in genomic DNA that is enriched by chromatin immunoprecipitation (ChIP). Alternate Names ChIP; ChIP Kit; ChIP-qPCR; Chromatin Immunopreciptiation; Chromatin IP; CnR; CUT & RUN; cut and run; CUT and RUN; CUT&RUN; CUTandrun; Luna; qPCR; Quantitive PCR; SYBR; Taq