CST, 89010T, Phospho-PINK1 (Ser228) (E9K3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PINK1 (Ser228) phosphate. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PINK1 (Ser228) (E9K3K) Rabbit Monoclonal Antibody (CST #89010) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PINK1 (Ser228) (E9K3K) Rabbit Monoclonal Antibody recognizes transfected levels of PINK1 protein only when phosphorylated at Ser228. Non-specific bands of unknown origin are observed migrating at ~32 kDa, and at ~80 kDa in mouse cell lines. Human species reactivity is weak by western blot. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser228 of human PINK1 protein. Background PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3). It has been shown that autophosphorylation of PINK1 at Ser228 and Ser402 is necessary for Parkin recruitment to damaged mitochondria (4). Alternate Names BRPK; FLJ27236; PARK6; PINK1; protein kinase BRPK; PTEN induced kinase 1; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 60, 50 Source/Isotype: Rabbit IgG