CST, 89011S, DNA Polymerase beta (F5I2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POLB. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DNA Polymerase beta (F5I2R) Rabbit Monoclonal Antibody (CST #89011) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DNA Polymerase beta (F5I2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNA polymerase β protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn245 of human DNA polymerase β protein. Background DNA polymerase β ( ) functions in the base excision repair (BER) DNA repair pathway, repairing single-strand DNA breaks (SSBs) arising from oxidative damage, deamination, alkylation, or abasic sites (1). In mitochondria, oxidative stress causes recruitment of DNA polymerase β to repair damaged mitochondrial DNA (mtDNA) (2). DNA polymerase β expression levels and mutations are associated with human cancer (3-6). Inhibition or downregulation of DNA polymerase β is synthetic lethal with defective mismatch repair (MMR) in models of acute lymphoblastic leukemia (ALL) (7) and with deficiency in models of breast cancer (8). Alternate Names DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; DPOLB; MGC125976; POLB; polymerase (DNA directed), beta; polymerase (DNA) beta Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG