CST, 89056T, STAU1 (E5Z1N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STAU1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, STAU1 (E5Z1N) Rabbit Monoclonal Antibody (CST #89056) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity STAU1 (E5Z1N) Rabbit Monoclonal Antibody recognizes endogenous levels of total STAU1 protein. This antibody recognizes both the 63 kDa (Uniprot #O95793-1) and 55 kDa (Uniprot #O95793-2) isoforms of STAU1. This antibody does not cross-react with STAU2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu486 of human STAU1 protein. Background Staufen is a multifunctional double-stranded RNA (dsRNA)-binding protein that was first described as a key regulator of development in early oocytes (1). Two Staufen orthologues are expressed in mammals, Staufen1 (STAU1) and Staufen2 (STAU2). Although both proteins share some sequence similarity, they have largely distinct spatiotemporal expression patterns and cellular functions (2). STAU1 is encoded by the gene and is ubiquitously expressed in most cell and tissue types. Multiple isoforms of STAU1 have been identified due to alternative splicing, with the two predominant isoforms being STAU1 and STAU1 (3,4). STAU1 plays a key role in multiple RNA processes, including splicing, translation, targeted mRNA decay (referred to as Staufen-mediated mRNA decay or SMD), and the transport of mRNA to subcellular regions during embryonic development and differentiation (1,5-10). STAU1 also transports mRNA along neuronal dendrites, contributing to localized protein synthesis and synaptic plasticity (11,12). Indeed, STAU1-deficient mice display reduced dendritic protrusions and impaired dendritic outgrowth, resulting in fewer synapses (13). STAU1 can undergo liquid-liquid phase separation and is frequently recruited to cytoplasmic foci known as stress granules (SGs), which are ribonucleoprotein (RNP) granules formed at sites of stalled mRNA translation (14,15). STAU1 has also been found to interact with ataxin-2, another SG-associated protein that is the primary cause of spinocerebellar ataxia type 2 (SCA2) when mutated (16). Cells derived from SCA2 patients display elevated levels of STAU1 protein and colocalization between STAU1 and ataxin-2 in SG-like aggregates (16). Recruitment of STAU1 to pathological protein aggregates has also been observed in other neurological disorders, such as amyotrophic lateral sclerosis (ALS) (17). STAU1 function has additionally been implicated in several types of cancer, including prostate, colorectal, and glioma (18-20).  Alternate Names Double-stranded RNA-binding protein Staufen homolog 1; FLJ25010; PPP1R150; protein phosphatase 1, regulatory subunit 150; STAU; STAU1; staufen double-stranded RNA binding protein 1; staufen, RNA binding protein, homolog 1; staufen, RNA binding protein, homolog 1 (Drosophila) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 63, 55 Source/Isotype: Rabbit IgG