CST, 89139S, SPIN1 (E6R1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Spindlin1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SPIN1 (E6R1Z) Rabbit Monoclonal Antibody (CST #89139) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SPIN1 (E6R1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPIN1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His33 of human SPIN1 protein. Background SPIN1, also known as Spindlin1, was first identified as a spindle binding protein during meiosis in mice (1). SPIN1 contains tandem tudor-like domains, which can recognize methylation of H3K4, H4K20, and H4R23 (2-4). SPIN1 localizes to rRNA genes to help drive their expression, and has also been shown to promote cellular proliferation through binding TCF4 to activate wnt signaling (2,5). This transcriptional activity is opposed by the SPIN1 docking protein SPINDOC, a repressor which causes SPIN1 to dissociate from chromatin (6). SPIN1 has shown to be overexpressed in ovarian cancers, and in other cell types SPIN1 overexpression causes cell cycle arrest and chromosome instability (7,8). SPIN1 has also been identified as a potential target in liposarcomas, where it contributes to RET signaling by controlling the expression of the activator GDNF (9). Alternate Names OCR; Ovarian cancer-related protein; SPIN; SPIN1; spindlin 1; Spindlin-1; Spindlin1; TDRD24 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG