CST, 89189S, Neuropilin-2 Antibody

Polyclonal Antibody for studying Neuropilin2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Neuropilin-2 Antibody (CST #89189) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Neuropilin-2 Antibody recognizes endogenous levels of total neuropilin-2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro681 of human neuropilin-2 protein. Antibodies are purified by peptide affinity chromatography. Background Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single-pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Neuropilin-2, a single-pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g., pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5). Alternate Names MGC126574; neuropilin 2; Neuropilin-2; neuropilin-2a(17); neuropilin-2a(22); neuropilin-2b(0); NP2; NPN2; NRP2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF165R2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120, 130 SOURCE: Rabbit