CST, 89217SF, FEN-1 (E5X2T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FEN-1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, FEN-1 (E5X2T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89217) is ready to ship. Product Usage Information This product is the carrier free version of product #83104. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 83104 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity FEN-1 (E5X2T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FEN-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to full-length human FEN-1 protein. Background Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double-stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10). Alternate Names DNase IV; FEN-1; FEN1; Flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1; RAD2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG