CST, 89270S, Helios (E4L5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Helios. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Helios (E4L5U) Rabbit Monoclonal Antibody (CST #89270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:125 - 1:500 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 59976 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Helios (E4L5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total Helios protein. Non-specific non-nuclear staining was observed in some mouse tissues by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly73 of human Helios protein. Background Helios (Ikaros family zinc finger 2, IKZF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4). Alternate Names ANF1A2; HELIOS; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; ZNFN1A2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 58, 63-70, 85 Source/Isotype: Rabbit IgG