CST, 89278SF, EMCN (E3Z4D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EMCN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, EMCN (E3Z4D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89278) is ready to ship. Product Usage Information This product is the carrier free version of product #96380. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 96380 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity EMCN (E3Z4D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EMCN protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human EMCN protein. Background Endomucin (EMCN) is a type I transmembrane O-sialoglycoprotein expressed in endothelium in venules and capillaries, but not arteries (1,2). EMCN prevents leukocyte adhesion in non-inflamed tissues, and downregulation of EMCN during inflammation facilitates migration of inflammatory cells into target tissues (3). Notch activation in endothelial cells downregulates EMCN and promotes migration of neutrophils, modulating acute inflammation in hepatic ischemia/reperfusion injury (4). EMCN deficiency in endothelial cells drives tumor lung metastasis by providing a premetastatic niche for cancer cell colonization, and is a potential prognostic biomarker for highly metastatic cancers (5). Combined analysis of EMCN/MUC15 has been suggested as a prognostic signature for gastric cancer (6). Alternate Names EMCN; EMCN2; Endomucin; Endomucin-2; Gastric cancer antigen Ga34; MUC-14; MUC14; MUCEN; Mucin-14 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95-110 Source/Isotype: Rabbit IgG