CST, 89426S, VE-Cadherin (D87F2) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying VE-Cadherin. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, VE-Cadherin (D87F2) Rabbit Monoclonal Antibody (PE Conjugate) (CST #89426) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity VE-Cadherin (D87F2) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total VE-cadherin protein. This antibody conjugate does not cross-react with other cadherin family proteins. Species Reactivity: Human, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VE-cadherin protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Alternate Names 7B4; 7B4 antigen; CADH5; cadherin 5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VE-cadherin Specification REACTIVITY: H B Pg SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG