CST, 89442W2, 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying TNFRSF9. Highly specific and rigorously validated in-house, 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #89442) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the extracellular domain of human 4-1BB/CD137/TNFRSF9 protein. Background TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6). Alternate Names 4-1BB; 4-1BB ligand receptor; CD137; CD137 antigen; CDw137; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; MGC2172; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; TNR9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG