CST, 8946S, TIMP1 (D10E6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIMP1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TIMP1 (D10E6) Rabbit Monoclonal Antibody (CST #8946) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1000 - 1:4000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76074 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TIMP1 (D10E6) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIMP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala134 of human TIMP1 protein. Background TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases (MMPs) that includes TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by directly binding to their catalytic zinc cofactor and resultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include TIMP1 with CD63, TIMP2 with α3β1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby's fundus dystrophy (8,9). Alternate Names CLGI; Collagenase inhibitor; EPA; epididymis secretory sperm binding protein; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP-1; TIMP1; tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG