CST, 89495S, PFKL (E7G5O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PFKL. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PFKL (E7G5O) Rabbit Monoclonal Antibody (CST #89495) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PFKL (E7G5O) Rabbit Monoclonal Antibody recognizes endogenous levels of total PFKL protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PFKL protein. Background Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2). The liver-type PFK (PFKL) is the predominant subunit of PFK found in the liver (1,3). Research studies have shown that because of its location on chromosome 21, this enzyme is overexpressed in Down Syndrome/trisomy 21 (3,4). PFKL also may have a role in the physiological response to a low fat diet (5). Alternate Names 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; K6PL; liver phosphofructokinase; liver-type 1-phosphofructokinase; PFK-B; PFK-L; PFKAL; PFKL; Phosphofructo-1-kinase isozyme B; Phosphofructokinase 1; phosphofructokinase, liver; phosphofructokinase, liver type; Phosphohexokinase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG