CST, 8954S, Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit Monoclonal Antibody mix

Monoclonal Antibody for studying . Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Tyrosine (P-Tyr-1000) MultiMab^® Rabbit Monoclonal Antibody mix (CST #8954) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24141 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Tyrosine (P-Tyr-1000) MultiMab Species Reactivity: All Species Expected Source / Purification MultiMab rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif. Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous MW (kDa): N/A Source/Isotype: Rabbit IgG