CST, 89555SF, IFI16 (D8B5T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IFI16. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, IFI16 (D8B5T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89555) is ready to ship. Product Usage Information This product is the carrier free version of product #14970. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14970 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IFI16 (D8B5T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IFI16 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro399 of human IFI16 protein. Background IFI16 belongs to the PYHIN family, which consists of four members in humans (Aim2, IFIX, MNDA, and IFI16) and at least 13 members in mice (1). The closest homolog of IFI16 in mice is p204. The PYHIN family is characterized by the presence of one or two HIN-200 domains that bind double-stranded DNA and a PYRIN domain that mediates interactions among family members, as well as the inflammasome-forming ASC protein (1). IFI16 also has a nuclear localization signal, but has been observed to bind DNA in both the cytoplasm and nucleus (2,3). There are three isoforms of IFI16 produced by alternative splicing (4). IFI16 is constitutively expressed by some cells, such as lymphocytes, and highly inducible in many others by interferons (5). IFI16 has been shown to directly interact with p53 and to play a role in cellular senescence by modulating p53 activity (6). IFI16 was shown to directly sense intracellular viral DNA, leading to recruitment of STING and activation of IRF-3 and NF-κB (2). Investigators have observed increased levels of IFI16 and anti-IFI16 autoantibodies in patients with systemic lupus erythematosus (7-9). Alternate Names Gamma-interferon-inducible protein 16; IF16; Ifi-16; IFI16; IFNGIP1; interferon gamma inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator; interferon, gamma-inducible protein 16; MGC9466; PYHIN2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85, 95, 98 Source/Isotype: Rabbit IgG