CST, 89572S, Galectin-3/LGALS3 (E7B6R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying galectin-3 mouse. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Galectin-3/LGALS3 (E7B6R) Rabbit Monoclonal Antibody (CST #89572) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 76015 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Galectin-3/LGALS3 (E7B6R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Galectin-3/LGALS3 protein. This antibody recognizes mouse Galectin-3/LGALS3 protein and is also reactive with human Galectin-3/LGALS3; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly15 of mouse Galectin-3/LGALS3 protein. Background Galectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-3/LGALS3 is involved in several diverse biological functions. Galectin-3/LGALS3 binds IgE (8). Galectin-3/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12). Alternate Names 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Gal-3; gal3; Galactose-specific lectin 3; galect; Galectin-3; GBP; IgE-binding protein; L-3; L-34; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactose binding, soluble 3; LEG3; Lgals3; Mac-; Mac-2; Mac-2 antigen Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG