CST, 8957S, Phospho-Rb (Ser807/811) (D20B12) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying Rb (Ser807/Ser811) phosphate. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Rb (Ser807/811) (D20B12) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #8957) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Rb (Ser807/811) (D20B12) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser807/811 of human Rb protein. Background The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9). Alternate Names exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG