CST, 8962S, PLA2G1B Antibody

Polyclonal Antibody for studying PLA2G1B. Validated for Western Blotting. Highly specific and rigorously validated in-house, PLA2G1B Antibody (CST #8962) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PLA2G1B Antibody recognizes endogenous levels of total PLA2G1B protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala131 of human PLA2G1B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Phospholipase A2 (PLA2) is a superfamily of enzymes that hydrolyze glycero-3-phosphocholines and release fatty acids and lysophospholipids (1). PLA2G1B is a member of this superfamily in the 1B group that is expressed most highly in the pancreatic acinar cells (2). Evidence suggests that PLA2G1B plays a role in the absorption and storage of extra energy as fats are metabolized (1,2). Lysophospholipids generated by PLA2G1B inhibit fatty acid oxidation in the liver and reduce energy expenditure, leading to diet-induced obesity and type 2 diabetes with a high fat diet (1). Therefore, a potential intervention of obesity and diabetes could target PLA2G1B in the digestive tract (2). Alternate Names Group IB phospholipase A2; MGC119834; MGC119835; PA21B; Phosphatidylcholine 2-acylhydrolase 1B; Phospholipase A2; phospholipase A2 group IB; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PLA2G1B; PPLA2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16 SOURCE: Rabbit