CST, 89633SF, ATGL (30A4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATGL. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, ATGL (30A4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89633) is ready to ship. Product Usage Information This product is the carrier free version of product #2439. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2439 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ATGL (30A4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total ATGL protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL. Background Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6). Alternate Names 1110001C14Rik; Adipose triglyceride lipase; ATGL; Calcium-independent phospholipase A2; Desnutrin; DKFZp667M109; FP17548; IPLA2-zeta; iPLA2zeta; mutant patatin-like phospholipase domain containing 2; patatin like phospholipase domain containing 2; patatin-like phospholipase domain containing 2; Patatin-like phospholipase domain-containing protein 2; PEDF-R; Pigment epithelium-derived factor; PLPL2; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS-2.2; TTS2; TTS2.2 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG