CST, 89650T, CDK2AP1 (E9D7P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDK2AP1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK2AP1 (E9D7P) Rabbit Monoclonal Antibody (CST #89650) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity CDK2AP1 (E9D7P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK2AP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human CDK2AP1 protein. Background Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was first identified as a tumor suppressor in oral cancer and is a member of the nucleosome remodeling and histone deacetylase (NuRD) complex (1-4). Also known as p12 or DOC-1, CDK2AP1 binds to unphosphorylated CDK2 and DNA polymerase alpha/primase to negatively regulate their activities (5,6). CDK2AP1 also plays a role in the silencing of Oct-4 during stem cell differentiation (7). Low CDK2AP1 expression in oral cancers is associated with poor prognosis (8). Lower expression has also shown to be resistant to cisplatin treatment (9). In addition to oral cancers, CDK2AP1 plays a role in other tumor types, including colorectal, testicular, and ovarian cancers (10,11). Alternate Names CDK2-associated protein 1; CDK2-associated protein 1; CDK2AP1; CDKA1; CDKAP1; cyclin dependent kinase 2 associated protein 1; cyclin-dependent kinase 2 associated protein 1; Cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer 1; Deleted in oral cancer-1; DOC-1; DOC1; DORC1; p12DOC-1; Putative oral cancer suppressor; ST19 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG