CST, 89661S, NCKIPSD (E6Z3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NckIPSD. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NCKIPSD (E6Z3N) Rabbit Monoclonal Antibody (CST #89661) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NCKIPSD (E6Z3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total NCKIPSD protein. This antibody is expected to recognize isoforms 1 and 3 of NCKIPSD protein. This antibody cross-reacts with a 60 kDa band of unknown origin by western blot in some cell lines. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val716 of human NCKIPSD protein. Background NCKIPSD (NCK-interacting protein with SH3 domain), also known as SPIN90, is a cytoskeletal adaptor protein involved in organization of the actin cytoskeleton and actin-based cell motility (1). Downregulation of NCKIPSD expression in breast cancer stroma is correlated with decreased survival. NCKIPSD regulates microtubule acetylation in cancer-associated fibroblasts (CAFs) (2). NCKIPSD also plays a role in modulating signaling in the central nervous system (3). Alternate Names 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3P21; dia interacting protein; Dia-interacting protein 1; diaphanous protein interacting protein; Diaphanous protein-interacting protein; DIP; DIP-1; DIP1; MGC23891; NCK interacting protein with SH3 domain; NCK-interacting protein with SH3 domain; NCKIPSD; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; SPIN90; SPN90; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; Wiskott-Aldrich syndrome protein-interacting protein Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG