CST, 8969T, Apaf-1 (D5C3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying APAF. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Apaf-1 (D5C3) Rabbit Monoclonal Antibody (CST #8969) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Apaf-1 (D5C3) Rabbit Monoclonal Antibody recognizes endogenous levels of total Apaf-1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro470 of human Apaf-1 protein. Background Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6). Alternate Names APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG