CST, 8977S, CLIP1/CLIP170 Antibody

Polyclonal Antibody for studying CLIP1/CLIP170. Validated for Western Blotting. Highly specific and rigorously validated in-house, CLIP1/CLIP170 Antibody (CST #8977) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CLIP1/CLIP170 Antibody recognizes endogenous levels of total CLIP1/CLIP170 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLIP1/CLIP170 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Microtubules (MTs) are polarized filaments composed of α/β tubulin heterodimers. The slower growing (minus) ends of MTs are located at centrosomes, and the faster growing (plus) ends extend into the cell periphery. Regulation of MT dynamics is important for multiple cellular functions, including cell division, migration, adhesion, membrane trafficking, and polarity (reviewed in 1). Cytoplasmic linker protein 1/170 (CLIP1/CLIP170) localizes to the plus ends of MTs (2), and binds to the Rac1/cdc42 effector protein IQGAP1. This complex is involved in establishing cell polarity (3). CLIP1/CLIP170 also facilitates MT-dependent organelle transport (4), and phosphorylation of CLIP1/CLIP170 by PLK1 and CK2 is required for efficient microtubule-kinetochore attachments in mitosis (5). Alternate Names CAP-GLY domain containing linker protein 1; CAP-Gly domain-containing linker protein 1; CLIP; CLIP-170; CLIP1; CLIP170; CYLN1; cytoplasmic linker 1; Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha-2; cytoplasmic linker protein CLIP-170; MGC131604; Reed-Steinberg cell-expressed intermediate filament-associated protein; Reed-Sternberg intermediate filament-associated protein; Restin; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein); RSN Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 170 SOURCE: Rabbit