CST, 89799S, Granzyme C Antibody

Polyclonal Antibody for studying Granzyme C mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Granzyme C Antibody (CST #89799) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme C Antibody recognizes endogenous levels of total Granzyme C protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val190 of mouse Granzyme C protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme C is the closest murine homolog of human Granzyme H and is located directly downstream of (4). Granzyme C has been demonstrated to induce cell death with a similar potency as Granzyme B, and CTLs from mice with reduced expression of Granzyme C and F display diminished cytotoxicity (5). Granzyme C has been identified as a marker for type 1 innate lymphoid cells (ILC1s) exhibiting cytotoxic effector function in addition to ‘helper-like' function (6). These cells have been shown to regulate antitumor immunity and autoimmunity in mouse models (6). Alternate Names AI323531; B10; CCP; CCP2; Ctl; Ctla; Ctla-5; Ctla5; Cytotoxic cell protease 2; GRAC; Granzyme C; Gzmc Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit