CST, 89871S, TACSTD2/TROP2 (F5P2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TACSTD2/TROP2 mouse. Validated for WB,IP,IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TACSTD2/TROP2 (F5P2T) Rabbit Monoclonal Antibody (CST #89871) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity TACSTD2/TROP2 (F5P2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TACSTD2/TROP2 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse TACSTD2/TROP2 protein. Background TROP2 is a transmembrane glycoprotein encoded by gene (tumor-associated calcium signal transducer 2). TROP2 was first discovered as a biomarker of invasive trophoblast cells and later reported in many types of cancer cells, in various organs during development, and adult stem cells during homeostasis (1,2). TROP2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain, and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site and PKC phosphorylation site (Ser303) (1-4). TROP2 functions by regulating multiple signaling pathways, including the interaction of its extracellular domain with integrin beta1 to regulate FAK signaling, the association of its transmembrane domain with claudin-1 and claudin-7 for tight junction formation, and the regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway (1,2,5-8). All these functions are important for its role in tumor proliferation, metastasis, and invasion (1,2). PKC can phosphorylate TROP2 at Ser303; the phosphorylation changes the cytoplasmic tail conformation and further promotes its signaling (9). TROP2 can be activated through intramembrane proteolysis first by TACE, followed by further cleavage by Presenilin 1 and Presenilin 2. The proteolysis process is required for its role in tumor cell proliferation (10,11). Alternate Names C80403; Cell surface glycoprotein Trop-2; EGP-; EGP-1; GA733-; GA733-1; Ly9; Ly97; lymphocyte antigen 97; MGC141612; MGC141613; TACD2; Tacstd2; Trop2; Tumor-associated calcium signal transducer 2; tunor-associated calcium signal transducer 2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 45-65 Source/Isotype: Rabbit IgG