CST, 8988T, DDX6/RCK (D26C11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX6/RCK (D26C11) Rabbit Monoclonal Antibody (CST #8988) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DDX6/RCK (D26C11) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX6/RCK protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala130 of human DDX6/RCK protein. Background DDX6, also known as RCK and p54, was identified as a proto-oncogene product and is a member of the ATP-dependent DEAD box helicase family (1,2). This protein interacts with translation initiation factor eIF4E in the cytoplasmic P-bodies (3) and represses mRNA translation (4). DDX6 is a component of the miRNA induced silencing complex (miRISC) and interacts with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) proteins and (5), functioning in miRNA-mediated translational repression (5). Depletion of DDX6 leads to the disruption of cytoplasmic P-bodies indicating that it is required for P-body formation (5). Alternate Names ATP-dependent RNA helicase p54; DDX6; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD-box helicase 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); FLJ36338; HLR2; IDDILF; Oncogene RCK; P54; Probable ATP-dependent RNA helicase DDX6; RCK; Rck/p54 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG