CST, 89954T, Estrogen Receptor beta (E2K7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ER-beta. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Estrogen Receptor beta (E2K7F) Rabbit Monoclonal Antibody (CST #89954) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Estrogen Receptor beta (E2K7F) Rabbit Monoclonal Antibody recognizes overexpressed levels of total estrogen receptor β protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu10 of human estrogen receptor β protein. Background Estrogen receptor β (ERβ) is part of the nuclear receptor superfamily related to estrogen receptor α (ERα) and encoded by the gene on chromosome 14 (1,2). Much like ERα, ERβ contains two activator functions, one in the N-terminal domain and one in the ligand binding domain. Both proteins have highly homologous DNA-binding domains and can synergistically regulate transcription (2,3). ERβ is tightly regulated by various mechanisms including estrogen binding, post-translational modifications, alternative splicing, circadian controls, and DNA methylation of the gene (4,5). The role of ERβ in various cancer types is controversial, but it is generally thought to be a tumor suppressor. Absence of ERβ has been shown to be a marker of poor prognosis in breast cancer and is correlated with tamoxifen resistance (6,7). In prostate cancer, ERβ can induce apoptosis, but its expression seems to be lost in higher grade tumors (8,9). Alternate Names ER-beta; Erb; ESR-BETA; ESR2; ESRB; ESTRB; estrogen receptor 2; estrogen receptor 2 (ER beta); Estrogen receptor beta; estrogen receptor beta 4; estrogen receptor beta splice variant, ERbeta2delta7; estrogen receptor beta splice variant, ERbeta4delta7; estrogen receptor beta splice variant, ERbeta6; estrogen receptor beta splice variant, ERbeta6delta7; estrogen receptor beta splice variant, ERbeta7; estrogen receptor beta splice variant, ERbeta7delta7; estrogen receptor beta splice variant, ERbetaEx. 4L; estrogen receptor beta splice variant, ERbetaEx. 6L; NR3A2; Nuclear receptor subfamily 3 group A member 2; ODG8; oestrogen receptor beta Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 60 Source/Isotype: Rabbit IgG