CST, 90039T, PKD/PKC mu (D4J1N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PKD1/PKCmu. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PKD/PKC mu (D4J1N) Rabbit Monoclonal Antibody (CST #90039) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PKD/PKC mu (D4J1N) Rabbit Monoclonal Antibody recognizes endogenous levels of total PKD/PKCμ protein. This antibody does not cross-react with human PKC α, β, γ, δ, ε, ζ, η, θ, ι proteins. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PKD/PKCμ protein. Background Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6). Alternate Names CHDED; KPCD1; nPKC-D1; nPKC-mu; PKC-MU; PKCM; PKD; PKD1; PRKCM; PRKD1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; protein kinase D1; Serine/threonine-protein kinase D1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit IgG