CST, 90051T, TRIM33 (D7U4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM33. Validated for WB,IP,IHC,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM33 (D7U4F) Rabbit Monoclonal Antibody (CST #90051) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6  cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Chromatin IP Specificity / Sensitivity TRIM33 (D7U4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM33 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln609 of human TRIM33 protein. Background The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain (1). Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability (2). TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex (3); it functions as a corepressor for Krüppel-associated box (KRAB) zinc-finger transcription factors (4). Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 (5). TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ (6). Alternate Names E3 ubiquitin-protein ligase TRIM33; ECTO; Ectodermin homolog; FLJ32925; KIAA1113; Protein Rfg7; PTC7; RET-fused gene 7 protein; RFG7; RING-type E3 ubiquitin transferase TRIM33; TF1G; TIF1-gamma; TIF1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; TRI33; TRIM33; tripartite motif containing 33; tripartite motif-containing 33; Tripartite motif-containing protein 33 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG