CST, 90173S, STING (E9X7F) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying STING. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, STING (E9X7F) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #90173) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:3200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity STING (E9X7F) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with human STING recombinant protein and reacts with an epitope near the carboxy terminus. Background Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). Alternate Names endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; SAVI; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; STING; STING-beta; STING1; TM173; TMEM173; Transmembrane protein 173 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG