CST, 90198T, BCKDH-E1 alpha (E4T3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCKDH E1-alpha. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BCKDH-E1 alpha (E4T3D) Rabbit Monoclonal Antibody (CST #90198) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BCKDH-E1 alpha (E4T3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCKDH-E1α protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCKDH-E1α protein. Background Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5). Alternate Names 2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; BCKDHA; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1 subunit alpha; branched chain keto acid dehydrogenase E1, alpha polypeptide; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; FLJ45695; MSU; MSUD1; ODBA; OVD1A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG