CST, 90219T, Phospho-BCL2L13 (Ser275) (F3K3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bcl-2L13 (Ser275) phosphate. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-BCL2L13 (Ser275) (F3K3M) Rabbit Monoclonal Antibody (CST #90219) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-BCL2L13 (Ser275) (F3K3M) Rabbit Monoclonal Antibody recognizes endogenous levels of BCL2L13 protein only when phosphorylated at Ser275. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser275 of human BCL2L13 protein. Background Bcl-2-like protein 13 (BCL2L13), also known as Bcl-rambo, is an atypical member of the Bcl-2 family of proteins that regulates apoptosis at the mitochondria (1). While it contains conserved Bcl-2 homology (BH) domains -1, -2, -3, and -4, it has a unique C-terminal region and does not interact with other Bcl-2 family members (1). BCL2L13 may regulate apoptosis in a cellular context manner (reviewed in 2). It was originally identified as a pro-apoptotic protein with activity dependent on its C-terminal region (1). Interaction with the adenine nucleotide translocator (ANT) suppresses the ADP/ATP-dependent translocation activity of ANT (3). Other studies have found that BCL2L13 can inhibit apoptosis in glioblastoma and tumor growth (4). These studies found that BCL2L13 binds to pro-apoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) and blocks homo- and heteromeric CerS2/6 complex formation and activity. Studies have also found that BCL2L13 can regulate mitochondrial fragmentation and can bind to LC3 to facilitate the clearance of damaged mitochondria through mitophagy (5,6). Phosphorylation of BCL2L13 at Ser275 plays a role in the clearance of damaged mitochondria through mitophagy and is important in maintaining cardiac function (7). Phosphorylation of BCL2L13 at Ser261 and Ser275 occurs following AMPK activation with Ser275 being controlled by TBK1. For more information, visit BioRxiv at https://www.biorxiv.org/content/10.1101/2025.07.10.663832v1 Alternate Names B2L13; Bcl-2-like protein 13; Bcl-2L13; Bcl-rambo; BCL2 like 13; Bcl2-L-13; BCL2-like 13 (apoptosis facilitator); BCL2L13; MIL1; Protein Mil1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG