CST, 90296S, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (HRP Conjugate) (CST #90296) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (HRP Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. This antibody conjugate recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich's Anti-FLAG antibodies. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1