CST, 90302SF, F4/80 (D4C8V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EMR1 mouse. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, F4/80 (D4C8V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #90302) is ready to ship. Product Usage Information This product is the carrier free version of product #30325. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 30325 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity F4/80 (D4C8V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total F4/80 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln260 of mouse F4/80 protein. Background F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4). Alternate Names Adgre1; Adhesion G protein-coupled receptor E1; AGRE1; Cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; TM7L; TM7LN3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 65-250 Source/Isotype: Rabbit IgG