CST, 90323SF, CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CCL3 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #90323) is ready to ship. Product Usage Information This product is the carrier free version of product #85270. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 85270 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CCL3/MIP-1α protein. This antibody detects a 42 kDa band of unknown origin in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CCL3/MIP-1α protein. Background Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α (MIP-1α), is a 10 kDa member of the C-C or β family of chemokines that play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 possesses hematopoietic stem cell inhibitory activity, suppressing their proliferation, but has been shown to promote proliferation of more mature progenitors (2-4). CCL3 has been implicated in several forms of leukemia, in which malignant cells may lose responsiveness to CCL3 or produce high levels of CCL3 (2). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (5,6). CCL3 expression is upregulated in the brains of patients with Alzheimer's disease (AD) with dementia, and reduced expression of CCL3 is observed in AD patients resilient to dementia (7). Alternate Names AI323804; C-C motif chemokine 3; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; Heparin-binding chemotaxis protein; L2G25B; LD78alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1-alpha; MIP-1alpha; MIP1; MIP1 (a); MIP1-(a); MIP1-alpha; Mip1a; Scya3; SIS-alpha; Small-inducible cytokine A3; TY-5 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG