CST, 90332S, SCARB1 (E9H4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCARB1 mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SCARB1 (E9H4F) Rabbit Monoclonal Antibody (CST #90332) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:150 - 1:600 Immunofluorescence (Frozen): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity SCARB1 (E9H4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total SCARB1 protein. Non-specific staining was observed in normal human small intestine by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile487 of mouse SCARB1 protein. Background Scavenger receptor class B member 1 (SCARB1, SR-B1) is a multi-ligand cell-surface receptor that facilitates uptake of lipids from cholesterol into cells (1,2). SCARB1 is part of the CD36 scavenger receptor superfamily, which is composed of many proteins with varied structures, expressions, and functions across tissue and cell types (1-3). SCARB1 recognizes a variety of ligands: high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS) (2,4,5). SCARB1 knockout mice have elevated HDL levels and increased susceptibility to atherosclerosis when given a high-fat diet, indicating its importance in the regulation of cholesterol homeostasis (6). Additionally, SCARB1 has a role in antigen cross-presentation from bacteria and viruses (1-3). SCARB1 has been reported to be an entry factor for the Hepatitis C virus (7). In endothelial cells and macrophages, SCARB1 has an anti-inflammatory and pro-survival role, which provides protection against atherosclerosis and helps to clear apoptotic cells (8). Alternate Names AI120173; CD36; Cd36l; Cd36l1; Chohd; Chohd1; Cla; Cla-1; Cla1; D5Ertd460; D5Ertd460e; HDL QTL 1; Hdlq; Hdlq1; Hlb39; Hlb398; mSR-BI; OTTMUSP00000026063; Scarb1; Scavenger receptor class B member 1; scavenger receptor class B type I; scavenger receptor class B, member 1; scavenger receptor class B1; SCRB1; SR-B1; SR-BI; SRB1; SRBI Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG