CST, 9033S, SPT5 Antibody

Polyclonal Antibody for studying SPT5. Validated for Western Blotting. Highly specific and rigorously validated in-house, SPT5 Antibody (CST #9033) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SPT5 Antibody recognizes endogenous levels of total SPT5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly803 of human SPT5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background DRB-sensitivity inducing factor (DSIF), a heterodimer composed of SPT4 and SPT5, is capable of both facilitating and inhibiting RNA polymerase II (RNAPII) activity (1-3). DSIF, together with NELF (Negative Elongation Factor), inhibits RNAPII elongation, resulting in promoter proximal pausing of RNAPII as it awaits additional signaling to resume transcription (4). The release of promoter proximal pausing is signaled through phosphorylation of the RNAPII C-terminal domain (CTD) and NELF by positive transcription elongation factor (P-TEFb) (5). P-TEFb also phosphorylates SPT5 at Thr4 within the evolutionarily conserved heptapeptide repeat motif. This phosphorylation event switches DSIF from a transcriptional repressor to an activator where it becomes a critical factor for transcriptional elongation (6,7). Alternate Names DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; DSIF large subunit; DSIF p160; FLJ34157; hSPT5; SPT5; SPT5 homolog, DSIF elongation factor subunit; SPT5H; suppressor of Ty 5 homolog; suppressor of Ty 5 homolog (S. cerevisiae); SUPT5H; Tat-cotransactivator 1 protein; Tat-CT1; Tat-CT1 protein; Transcription elongation factor SPT5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit