CST, 90370T, DDX42 (F4V2L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX42. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DDX42 (F4V2L) Rabbit Monoclonal Antibody (CST #90370) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DDX42 (F4V2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX42 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human DDX42 protein. Background DEAD box protein 42 (DDX42) is an ATP-dependent DEAD box RNA helicase that has key functions in splicing. DDX42 has RNA chaperone activity and associates with the SF3b complex along with DDX46, facilitating 17S U2 snRNP assembly (1-4). In oncogenic mutations, interactions between SF3B1 and DDX42/46 are greatly reduced, resulting in alternative splicing (5). DDX42 also plays a key role in the inhibition of positive-strand RNA viruses, including HIV and SARS-CoV-2 (6,7). In addition to its role in splicing, DDX42 is capable of binding G-quadruplexes, higher-order structures in the genome which can be aberrantly accumulated in cancer (8). Alternate Names ATP-dependent RNA helicase DDX42; DDX42; DDX42P; DEAD (Asp-Glu-Ala-Asp) box helicase 42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; DEAD-box helicase 42; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; SF3b125; SF3b125 DEAD-box protein; SF3B8; Splicing factor 3B-associated 125 kDa protein; splicing factor 3b, subunit 8 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG