CST, 90389T, TNIK (F7V3J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNIK. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, TNIK (F7V3J) Rabbit Monoclonal Antibody (CST #90389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TNIK (F7V3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total TNIK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg567 of human TNIK protein. Background TRAF2 and NCK-interacting protein kinase (TNIK) is a member of the germinal center kinase (GCK) family (1). TNIK phosphorylates TCF4 and is an essential activator for Wnt signaling (2). Animal knockout model and kinase inhibition studies have reported that TNIK can stimulate both cancer cell growth and epithelial-mesenchymal transition (EMT) (3-5). TNIK has also been shown to promote F-actin disruption through its interactions with Rap2 (6). In neuronal cells, TNIK is enriched in the postsynaptic density (PSD), where it is reported to modulate neuronal receptor surface expression and dendrite complexity and signaling (7-9). Alternate Names KIAA0551; MRT54; TNIK; TRAF2 and NCK interacting kinase; TRAF2 and NCK-interacting protein kinase; ZC2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG