CST, 9038S, Cell Fractionation Kit

Buffer Kit for studying in the research area. Storage Store at -20°C. Upon receipt, Protease Inhibitor Cocktail 5871 should be stored at 4°C. All other components should be stored at -20°C. Protocol Available protocols: Western Blotting, Functional Background Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4). Alternate Names Cellular; cytoplasmic; cytoskeletal; Fractionation; membrane; nuclear; organelle; organelle marker; subcellular