CST, 90393S, S100B (E7C3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying S100B. Validated for WB,WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, S100B (E7C3A) Rabbit Monoclonal Antibody (CST #90393) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity S100B (E7C3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100B protein. This antibody recognizes human S100B protein and is also reactive with mouse S100B; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His86 of human S100B protein. Background Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes, such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cations in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). S100B is abundantly expressed in astrocytes and is commonly used as an astrocytic marker in studies of the mammalian CNS. S100B is also expressed in immature and mature myelinating oligodendrocytes that are chondroitin sulfate proteoglycan (NG2)-positive (5). Alternate Names NEF; Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 beta; S100 calcium binding protein B; S100 calcium binding protein, beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100-B; S100B; S100beta Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG