CST, 90394T, CaMKII-gamma (E4C3O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CaMK2-gamma. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CaMKII-gamma (E4C3O) Rabbit Monoclonal Antibody (CST #90394) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CaMKII-gamma (E4C3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CaMKII-γ protein. This antibody does not cross-react with CaMKII-α, CaMKII-β, or CaMKII-δ proteins. This antibody non-specifically labels colon by Immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu350 of human CaMKII-γ protein. Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca /calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4). Alternate Names calcium/calmodulin dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II gamma; Calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CAMK; CAMK-II; CaMK-II subunit gamma; CAMK2G; CAMKG; CaMKII; FLJ16043; KCC2G; MGC26678; MRD59 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG