CST, 9039T, NDRG4 (D4A6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NDRG4. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, NDRG4 (D4A6) Rabbit Monoclonal Antibody (CST #9039) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Frozen): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49334 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity NDRG4 (D4A6) Rabbit Monoclonal Antibody recognizes endogenous levels of total NDRG4 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly217 of human NDRG4 protein. Background The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5). Unlike other members of the family that are widely expressed, NDRG4/Bdm1 expression is restricted primarily to the heart and brain (2,3,6,7). Multiple alternatively spliced isoforms of NDRG4 have been reported (3,7). Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease (3). In contrast, NDRG4 expression is elevated in glioblastoma and contributes to cell cycle progression and survival (8). NDRG4 also may be inactivated by promotor methylation in colorectal cancer and function as a tumor suppressor gene (9). Alternate Names BDM1; Brain development-related molecule 1; DKFZp686I1615; FLJ30586; FLJ42011; KIAA1180; MGC19632; N-myc downstream-regulated gene 4 protein; NDRG family member 4; NDRG4; Protein NDRG4; SMAP-8; SMAP8; smooth muscle-associated protein 8; Vascular smooth muscle cell-associated protein 8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 37-45 Source/Isotype: Rabbit IgG