CST, 90426S, POR (F6Y3C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cytochrome P450 Reductase. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, POR (F6Y3C) Rabbit Monoclonal Antibody (CST #90426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity POR (F6Y3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total POR protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POR protein. Background Cytochrome P450 oxidoreductase (POR), also known as CYPOR, is a diflavin reductase that uses flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) as cofactors and nicotinamide adenine dinucleotide phosphate (NADPH) as an electron donor (reviewed in 1). Cytochrome P450 is a family of heme-containing enzymes that function as monooxygenases, oxidizing steroids, fatty acids, drugs, and xenobiotics. These enzymes all depend on POR for electron supply. In addition, POR donates electrons to heme oxygenase, cytochrome b5, and cytochrome c. In addition to its various roles in drug and steroid metabolism, studies have found that POR contributes to lipid peroxidation during ferroptosis (2-4). Alternate Names CPR; CYPOR; cytochrome p450 oxidoreductase; DKFZp686G04235; FLJ26468; NADPH--cytochrome P450 reductase; NADPH--hemoprotein reductase; NADPH-dependent cytochrome P450 reductase; NCPR; P450 (cytochrome) oxidoreductase; P450R; POR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG